Journal: 2D Materials

Article Title: Positive resolution of the wound-healing response in lens epithelial cells by Ti₃C₂T _x MXene coatings for use in accommodative intraocular lens devices

doi: 10.1088/2053-1583/ac95a7

Figure Lengend Snippet: Figure 4. Human lens epithelial (HLE-B3) cells grown on uncoated and Ti3C2Tx-coated tissue grade 12-well plates. Wound closure rate was quantified using light microscope images taken at the selected time points (figure (A)). Light microscope images of HLE-B3 cells grown on uncoated (figures (B) and (D)) and Ti3C2Tx-coated (figures (C) and (E)) stained with crystal violet at t = 0 h (top) and t = 120 h (bottom). x50 magnification by light microscope (Zeiss Primovert HDcam, Germany). Scale bar: 25 µM. Measurements were calculated using Fiji Image J. Wound closure was calculated as the width difference in the scratch across the cell monolayer at t = 0 h relative to the other time points. A two-way ANOVA was completed using GraphPad Prism 5. ∗∗p < 0.01; ∗∗∗p < 0.001. (Uncoated: cells grown on tissue culture well; Ti3C2Tx-coated: cells grown on Ti3C2Tx-coated wells) (Mean ± SEM; n = 6).

Article Snippet: Images were captured at 0, 24, 48, 72 and 120 h using a Zeiss Primovert HDcam (Carl Zeiss Microscopy GmbH, Germany).

Techniques: Light Microscopy, Staining

Journal: Experimental cell research

Article Title: E6/E7 from Beta-2-HPVs 122, 38b, and 107 possess transforming properties in a fibroblast model in vitro.

doi: 10.1016/j.yexcr.2022.113088

Figure Lengend Snippet: Fig. 1. Representative images of the microscopic photo-documentation of the cell models generated over time. Microscopic observations of FB-pLVX (primary fibroblast transduced with empty vector), or with E6/E7 of HPVs 16, 18, 38b, 107, and 122. The photographs are representative of passages 8, 12, 15, and 20, taken with an inverted microscope (40X amplification). The skull symbol means that the cells no longer survived that passage.

Article Snippet: Photographs were obtained with 4× objective at 0, 6, 12, and 24 h with the use of a ZEISS inverted microscope (Model Primo Vert No. 415510-1101-000, Carl Zeiss microscopy GmbH, Jena, Germany), with a ZEISS AxioCam camera (Model ERc5s, Carl Zeiss microscopy GmbH, Jena, Germany), using the ZEN 2012 software (Blue edition, Carl Zeiss microscopy GmbH, Jena, Germany).

Techniques: Generated, Transduction, Plasmid Preparation, Inverted Microscopy, Amplification